Aminopeptidase N is located in the small-intestinal and renal microvillar membrane, and also in other plasma membranes. In the small intestine aminopeptidase N plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. Its function in proximal tubular epithelial cells and other cell types is less clear. The large extracellular carboxyterminal domain contains a pentapeptide consensus sequence characteristic of members of the zinc-binding metalloproteinase superfamily. Sequence comparisons with known enzymes of this class showed that CD13 and aminopeptidase N are identical. The latter enzyme was thought to be involved in the metabolism of regulatory peptides by diverse cell types, including small intestinal and renal tubular epithelial cells, macrophages, granulocytes, and synaptic membranes from the CNS. Human aminopeptidase N is a receptor for one strain of human coronavirus that is an important cause of upper respiratory tract infections. Defects in this gene appear to be a cause of various types of leukemia or lymphoma.

Target	Aminopeptidase N (ANPEP)
Clonality	Monoclonal
Reactivity	Human
Tested Applications	ELISA, WB
Host	Mouse
Recommended dilutions	WB: 1/500 - 1/1000. Optimal dilutions/concentrations should be determined by the end user.
Conjugation	Unconjugated
Immunogen	Purified His-tagged Human ANPEP protein (Fragment)
Isotype	IgG1
Form	Liquid
Purification	Purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Storage	Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
UniProt Primary AC	P15144 (UniProt, ExPASy)
UniProt Secondary AC	Q16728, Q6GT90, Q8IUK3, Q8IVH3, Q9UCE0
UniProt Entry Name	AMPN_HUMAN
KEGG	hsa:290
String	9606.ENSP00000300060
Molecular Weight	Calculated MW: 110 kDa
Buffer	PBS containing 0.09% sodium azide.
Availability	Shipped within 5-10 working days.
Note	This product is for research use only.

Research Articles on Aminopeptidase N (ANPEP)